Evaluation of DNA Damage in Human Lymphocytes Exposed to Triclosan by Alkaline Comet Assay
DOI:
https://doi.org/10.70749/ijbr.v3i7.2121Keywords:
Triclosan, genotoxicity, antimicrobial agent, lymphocytesAbstract
In the present study, the in-vitro effect of Triclosan was tested on human peripheral lymphocytes taken in different concentrations. The genotoxicity of these antimicrobial agents was assessed using alkaline comet assay. The lymphocytes were isolated from the blood of non-smoker healthy students, which were treated with different concentrations of the selected antimicrobial agent i.e. Triclosan (2.5mg/mL, 4mg/mL, 5mg/mL, 6.5mg/mL, 7.5mg/Ml, 10mg/mL) and were kept for 1 hour in a dry bath incubator. The result demonstrated that Triclosan was able to induce DNA damage with all concentrations. The amount of damage was directly proportional to the concentration of antimicrobial drugs used.
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1. Ciofu O, Moser C, Jensen PØ, Høiby N. Tolerance and resistance of microbial biofilms. Nature Reviews Microbiology. 2022;20(10):621-35. https://doi.org/10.1038/s41579-022-00682-4
2. Mertens RT, Gukathasan S, Arojojoye AS, Olelewe C, Awuah SG. Next generation gold drugs and probes: chemistry and biomedical applications. Chemical reviews. 2023;123(10):6612-67. https://doi.org/10.1021/acs.chemrev.2c00649
3. Luo Y, Song Y. Mechanism of antimicrobial peptides: antimicrobial, anti-inflammatory and antibiofilm activities. International journal of molecular sciences. 2021;22(21):11401. https://doi.org/10.3390/ijms222111401
4. Uddin TM, Chakraborty AJ, Khusro A, Zidan BRM, Mitra S, Emran TB, et al. Antibiotic resistance in microbes: History, mechanisms, therapeutic strategies and future prospects. Journal of infection and public health. 2021;14(12):1750-66. https://doi.org/10.1016/j.jiph.2021.10.020
5. Alfhili MA, Lee M-H. Triclosan: an update on biochemical and molecular mechanisms. Oxidative medicine and cellular longevity. 2019;2019(1):1607304. https://doi.org/10.1155/2019/1607304
6. Ladeira C, Møller P, Giovannelli L, Gajski G, Haveric A, Bankoglu EE, et al. The comet assay as a tool in human biomonitoring studies of environmental and occupational exposure to chemicals—a systematic scoping review. Toxics. 2024;12(4):270. https://doi.org/10.3390/toxics12040270
7. Duarte NdAA, Lima LEd, Maraslis FT, Kundi M, Nunes EA, Barcelos GRM. Acute toxicity and DNA instability induced by exposure to low doses of triclosan and phthalate DEHP, and their combinations, in vitro. Frontiers in genetics. 2021;12:649845. https://doi.org/10.3389/fgene.2021.649845
8. Gonçalves S, Gaivão I. Natural ingredients common in the Trás-Os-Montes region (Portugal) for use in the cosmetic industry: a review about chemical composition and antigenotoxic properties. Molecules. 2021;26(17):5255. https://doi.org/10.3390/molecules26175255
9. Arenaz P. Chinese hamster apurinic/apyrimidinic endonuclease (chAPE1) expressed in sf9 cells reveals that its endonuclease activity is regulated by phosphorylation. The FEBS Journal. 2025. https://doi.org/10.1111/j.1742-4658.2010.07879.x
10. Jiang N, Naz S, Ma Y, Ullah Q, Khan MZ, Wang J, et al. An overview of comet assay application for detecting DNA damage in aquatic animals. Agriculture. 2023;13(3):623. https://doi.org/10.3390/agriculture13030623
11. Gupta P, Makkar TK, Goel L, Pahuja M. Role of inflammation and oxidative stress in chemotherapy-induced neurotoxicity. Immunologic Research. 2022;70(6):725-41. https://doi.org/10.1007/s12026-022-09307-7
12. Muruzábal-Gambarte D. Optimization of the in vitro comet assay as a tool for mechanistic risk assessment. 2021.
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