Nutritional Assessment of Fermented Almond Milk Fortified with Vitamin B12

Authors

  • Rimsha Nazir National Institute of Food Science and Technology (NIFSAT), Agriculture University Faisalabad, Pakistan
  • Maryam Ashraf National Institute of Food Science and Technology (NIFSAT), Agriculture University Faisalabad, Pakistan
  • Khadija Mushtaq Bahuddin Zakariya University, Multan, Pakistan
  • Laiba Rauf Department of Human Nutrition and Dietetics, Superior College, Mian Channu, Pakistan
  • Javeria Qayyum Department of Human Nutrition and Dietetics, Superior College, Mian Channu, Pakistan
  • Kashifa Mehmood Department of Human Nutrition and Dietetics, Superior College, Mian Channu, Pakistan
  • Muhammad Usama Department of Human Nutrition and Dietetics, Superior College, Mian Channu, Pakistan
  • Samar Ijaz National Institute of Food Science and Technology (NIFSAT), Agriculture University Faisalabad, Pakistan
  • Maria Haris University of Central Punjab, Lahore, Pakistan

DOI:

https://doi.org/10.70749/ijbr.v3i8.2243

Keywords:

Fermented almond milk, fortification of vitamin B12, malnutrition, functional foods, almond milk production

Abstract

Dietary patterns have been drastically changed by sedentary behaviors and changes in lifestyle, which has increased nutritional imbalances in a number of populations. The ability of functional meals to deliver vital nutrients with minimal adverse effects has led to their widespread acceptance as a natural substitute for traditional medications. Because of its rich nutritional profile and health-promoting qualities, almond milk has become a possible plant-based substitute for other functional beverages. Notwithstanding these advantages, vitamin B12, a necessary ingredient for DNA synthesis, neurological function, and red blood cell development, is absent from almond milk. Developing fermented almond milk supplemented with vitamin B12 and assessing its nutritional makeup, mineral profile, antioxidant activity, and consumer acceptability were the goals of the current study. Probiotic cultures were used for fermentation, and standardized quantities of vitamin B12 were added for fortification. Fortified samples outperformed unfortified controls in terms of nutrition, according to proximate composition and mineral analysis. As fermentation progressed, antioxidant activity rose dramatically, and sensory analysis verified that the enriched product was well-received in terms of taste, color, texture, and general palatability. According to the research, vitamin B12-fortified fermented almond milk may be a sustainable, health-promoting beverage that is especially advantageous for vegetarians, people with lactose intolerance, and groups at risk of micronutrient shortages.

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References

1. Alozie, Y., & Udofia, U. (2015). Nutritional and sensory properties of almond and soy milk. Journal of Food Science and Nutrition, 3(2), 45–52.

2. Asim, M., & Nawaz, Y. (2018). Child malnutrition in Pakistan: Evidence from literature. Children, 5(5), 60.

https://doi.org/10.3390/children5050060

3. Bernart, A., et al. (2015). Probiotic fermentation of almond milk: Bioactive properties and health implications. Journal of Functional Foods, 13, 1–9.

4. Bernstein, M. A. (2017). Micronutrient malnutrition: Global health challenges. Nutrition Reviews, 75(1), 21–32.

5. Catarino, M. D., et al. (2018). Nutritional profile and bioactive compounds of almonds. Food Chemistry, 256, 259–265.

6. Demir, E., et al. (2016). Consumer acceptance of plant-based milk alternatives: A comparative study. Food Quality and Preference, 49, 30–40.

7. Ojo, O., Wang, X. H., Ojo, O. O., & Adegboye, A. R. A. (2021). The effects of almonds on gut microbiota, glycometabolism, and inflammatory markers in patients with type 2 diabetes: a systematic review and meta-analysis of randomised controlled trials. Nutrients, 13(10), 3377.

https://doi.org/10.3390/nu13103377

8. Kundu, P., et al. (2018). Plant-based milk substitutes: Processing and functional properties. Critical Reviews in Food Science and Nutrition, 58(10), 1524–1531.

9. Landstrom, E., et al. (2009). Functional foods: Consumer perceptions and health effects. British Journal of Nutrition, 101(6), 843–864.

10. Prytulska, N., Motuzka, I., Koshelnyk, A., Motuzka, O., Yashchenko, L., Jarossová, M., ... & Habánová, M. (2021). Consumer preferences on the market of plant-based milk analogues.

https://doi.org/10.5219/1485

11. Manzoor, M. F., et al. (2021). Effect of thermosonication on quality of almond milk. Ultrasonics Sonochemistry, 70, 105299.

12. Sethi, S., Tyagi, S. K., & Anurag, R. K. (2016). Plant-based milk alternatives: An emerging segment of functional beverages. Critical Reviews in Food Science and Nutrition, 56(3), 340–349.

https://doi.org/10.1007/s13197-016-2328-3

13. UNICEF. (2020). State of the World’s Children 2020: Children, food and nutrition. United Nations Children’s Fund.

14. Asim, M., & Nawaz, Y. (2018). Child malnutrition in Pakistan: Evidence from literature. Children, 5(5), 60.

https://doi.org/10.3390/children5050060

15. Reyes-Jurado, F., Soto-Reyes, N., Dávila-Rodríguez, M., Lorenzo-Leal, A. C., Jiménez-Munguía, M. T., Mani-López, E., & López-Malo, A. (2023). Plant-based milk alternatives: Types, processes, benefits, and characteristics. Food Reviews International, 39(4), 2320-2351.

https://doi.org/10.1080/87559129.2021.1952421

16. Bernart, A., Pinelo, M., Meyer, A. S., & Nielsen, C. R. (2015). Probiotic fermentation of plant-based milk alternatives: Bioactive properties and health implications. Journal of Functional Foods, 13, 1–9.

https://doi.org/10.1016/j.jff.2014.12.021

17. Bernstein, M. A. (2017). Micronutrient malnutrition: Global health challenges. Nutrition Reviews, 75(1), 21–32.

https://doi.org/10.1093/nutrit/nuw048

18. Catarino, M. D., Silva, A. M., & Cardoso, S. M. (2018). Almonds: Nutritional profile, bioactive compounds, and health benefits. Food Chemistry, 256, 259–265.

https://doi.org/10.1016/j.foodchem.2018.02.142

19. Demir, E., Aydemir, T., & Ozcan, T. (2016). Consumer perception and sensory evaluation of plant-based milk alternatives. Food Quality and Preference, 49, 30–40.

https://doi.org/10.1016/j.foodqual.2015.11.004

20. Government of Pakistan (GOP). (2018). National Nutrition Survey of Pakistan 2018. Ministry of Planning, Development & Reform, Islamabad, Pakistan.

21. Landstrom, E., Hursti, U. K., Magnusson, M., & Koivisto Hursti, U. K. (2009). Functional foods: Consumer perceptions and health effects. British Journal of Nutrition, 101(6), 843–864.

https://doi.org/10.1017/S0007114508059027

22. Lipan, L., Toldrá, F., & Mora, L. (2021). Plant-based milk substitutes: Market trends, composition, and nutritional value. Food Research International, 140, 109878.

https://doi.org/10.1016/j.foodres.2020.109878

23. AOAC. (2019). Official Methods of Analysis of AOAC International (21st ed.). AOAC International, Arlington, USA.

24. Bernat, N., Cháfer, M., Chiralt, A., & González-Martínez, C. (2014). Probiotic fermented almond “milk” as an alternative to cow-milk yoghurt. International Journal of Food Studies, 3(1), 23–33.

https://doi.org/10.7455/ijfs/4.2.2015.a8

25. Meilgaard, M., Civille, G. V., & Carr, B. T. (2016). Sensory evaluation techniques (5th ed.). CRC Press.

26. Modupe, A. E., & Diosady, L. L. (2021). Vitamin fortification of plant-based beverages: A review of technology and stability. Journal of Food Science and Technology, 58(7), 2432–2444.

27. Montgomery, D. C. (2017). Design and analysis of experiments (9th ed.). Wiley.

28. Ozturkoglu-Budak, S., Akal, C., & Yetisemiyen, A. (2016). Effect of storage on the survival of probiotics in fermented almond milk. LWT - Food Science and Technology, 68, 363–369.

https://doi.org/10.1016/j.lwt.2015.12.010

29. Reyes, R. G., Garcia, L. J., & Zamora, E. (2021). Development of almond milk as an alternative dairy beverage: Nutritional and functional aspects. Food Research International, 143, 110279.

https://doi.org/10.1016/j.foodres.2021.110279

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Published

2025-08-30

How to Cite

Nazir, R., Ashraf, M., Mushtaq, K., Rauf, L., Qayyum, J., Mehmood, K., Muhammad Usama, Ijaz, S., & Haris, M. (2025). Nutritional Assessment of Fermented Almond Milk Fortified with Vitamin B12. Indus Journal of Bioscience Research, 3(8), 523-527. https://doi.org/10.70749/ijbr.v3i8.2243