Quantitative Determination of Lidocaine Hydrochloride in Pharmaceutical Preparations Using HPLC-UV Detection

Nadeem Ul Hassan Khan; Asad Hamad; Quratulain; Naveed Suleman; Sana Rashid; Abdul Sami; Muhammad Aamir Nawaz

doi:10.70749/ijbr.v3i1.494

Authors

Nadeem Ul Hassan Khan Department of Forensic Medicine, University of Health Sciences, Lahore, Punjab, Pakistan.
Asad Hamad Faculty of Pharmacy, Grand Asian University, Sialkot, Punjab, Pakistan.
Quratulain Department of Pharmaceutical Chemistry, Imran Idrees College of Pharmacy, Sialkot, Punjab, Pakistan.
Naveed Suleman Department of Pharmacology, University of Health Sciences, Lahore, Punjab, Pakistan.
Sana Rashid Riphah Institute of Pharmaceutical Sciences, Riphah International University, Lahore, Punjab, Pakistan.
Abdul Sami Institute of Molecular Biology and Biotechnology, The University of Lahore, Punjab, Pakistan.
Muhammad Aamir Nawaz Faculty of Pharmacy, Gomal University, Dera Ismail Khan, KP, Pakistan.

DOI:

https://doi.org/10.70749/ijbr.v3i1.494

Keywords:

Lidocaine Hydrochloride, HPLC-UV, Quantification, Pharmaceutical Analysis, Quality Control, Local Anesthetics

Abstract

Objective: To develop and validate a robust and sensitive High-Performance Liquid Chromatography (HPLC) method with ultraviolet (UV) detection for the quantification of Lidocaine-HCl in pharmaceutical formulations. Methods: An Ion Pac Ercus C18 reversed-phase column (250 mm × 4.5 mm, 5 µm) was used at 25°C with a mobile phase of water and acetonitrile (80:20, v/v) containing 5% acetic acid (pH 3.4). The flow rate was maintained at 1.0 mL/min, and detection was performed at 254 nm. Calibration standards (0.1–0.5 µg/mL) were prepared, and validation parameters including linearity, sensitivity, precision, and accuracy were assessed. Recovery studies were performed using spiked commercial samples. Results: The method showed excellent linearity (R² = 0.9987). LLOD and LLOQ were 0.00521 µg/mL and 0.01645 µg/mL, respectively. Intra- and inter-day precision had RSD values ≤0.57%. Recovery ranged from 96% to 100%. Retention time was consistent at 12.5 minutes. Conclusion: This validated HPLC-UV method is precise, sensitive, and reliable for routine quantification of Lidocaine-HCl, ensuring pharmaceutical quality control.

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