The Toxicological Effects of Glyphosate on Hematological and Genotoxic Parameters in Coturnix japonica (Japanese Quail)
DOI:
https://doi.org/10.70749/ijbr.v3i7.1705Keywords:
Toxicological Effects, Glyphosate, Hematological Parameters, Genotoxic ParametersAbstract
Significant ecological and environmental issues have emerged due to the increased use of agrochemicals to meet the food demands of a growing population. In the agricultural industry, glyphosate is widely applied to various food crops. However, prolonged exposure to this chemical has been associated with serious endocrine-disrupting effects in animals, along with potential health implications for humans. This study aimed to evaluate the hematological and genotoxic effects of glyphosate in Japanese quail (Coturnix japonica). A total of 18 adult quails were obtained and randomly divided into four groups (A–D). Glyphosate was administered orally for 21 days. Group A served as the control, while groups B, C, and D received glyphosate at doses of 334.0 mg/kg, 33.40 mg/kg, and 3.340 mg/kg body weight, respectively. The results revealed significant hematological abnormalities, including anemia, thrombocytopenia, and oxidative stress. Red blood cell (RBC) count and hemoglobin (Hb) levels showed a marked decline over time in the higher exposure groups. These findings corroborate earlier studies indicating glyphosate's toxic effects across various species and underscore the potential ecological risks posed by its widespread use.
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1. Schreinemachers, P., et al., Can public GAP standards reduce agricultural pesticide use? The case of fruit and vegetable farming in northern Thailand. Agriculture and Human Values, 2012. 29(4): p. 519-529. https://link.springer.com/article/10.1007/s10460-012-9378-6
2. Bernhardt, E.S., E.J. Rosi, and M.O. Gessner, Synthetic chemicals as agents of global change. Frontiers in Ecology and the Environment, 2017. 15(2): p. 84-90. https://doi.org/10.1002/fee.1450
3. Shattuck, A., et al., Global pesticide use and trade database (GloPUT): New estimates show pesticide use trends in low-income countries substantially underestimated. Global Environmental Change, 2023. 81: p. 102693. https://doi.org/10.1016/j.gloenvcha.2023.102693
4. Singh, N.S., et al., Pesticide contamination and human health risk factor, in Modern age environmental problems and their remediation2017, Springer. p. 49-68.
5. Rani, M. and U. Shanker, Highly efficient removal of endocrine disrupting pesticides by metal ferrites loaded Guar gum based green nanomaterials. Journal of Molecular Liquids, 2023. 387: p. 122611. https://doi.org/10.1016/j.molliq.2023.122611
6. Olayemi, O.A., Comparative toxicity of two different pesticides on the skin of Japanese quail (Cortunix japonica). World's Veterinary Journal, 2015. 5(1): p. 13-18. https://doi.org/10.5455/wvj.20150248
7. Mitra, A., C. Chatterjee, and F.B. Mandal, Synthetic chemical pesticides and their effects on birds. Res J Environ Toxicol, 2011. 5(2): p. 81-96. https://doi.org/10.3923/rjet.2011.81.96
8. Vannini, A., et al., Bioaccumulation, physiological and ultrastructural effects of glyphosate in the lichen Xanthoria parietina (L.) Th. Fr. Chemosphere, 2016. 164: p. 233-240. https://doi.org/10.1016/j.chemosphere.2016.08.058
9. Pan XingLu, P.X., et al., Progress of the discovery, application, and control technologies of chemical pesticides in China. 2019. https://doi.org/10.1016/s2095-3119(18)61929-x
10. Peillex, C. and M. Pelletier, The impact and toxicity of glyphosate and glyphosate-based herbicides on health and immunity. Journal of immunotoxicology, 2020. 17(1): p. 163-174. https://doi.org/10.1080/1547691x.2020.1804492
11. Sarfraz, A., et al., Pre-Hatch Growth and Development of Selected Internal Organs of Japanese Quail (Coturnix japonica). Pakistan Veterinary Journal, 2019. 39(3).
https://doi.org/10.29261/pakvetj/2019.071
12. Qureshi, N., et al., Feeding habits of common quail (Coturnix coturnix) migrating through Rawalpindi, Pakistan. Pakistan Journal of Zoology, 2012. 44(6).
13. Ritchie, A., Ecological and genetic indicators of restoration success. 2015.
14. Djaber, N., et al., Roundup-induced biochemical and histopathological changes in the liver and kidney of rats: the ameliorative effects of Linum usitatissimum oil. Acta Biochimica Polonica, 2020. 67(1): p. 53-64. https://doi.org/10.18388/abp.2020_2898
15. Panzacchi, S., et al., The Ramazzini Institute 13-week study on glyphosate-based herbicides at human-equivalent dose in Sprague Dawley rats: study design and first in-life endpoints evaluation. Environmental Health, 2018. 17(1): p. 52. https://doi.org/10.1186/s12940-018-0393-y
16. Székács, A. and B. Darvas, Forty years with glyphosate. Herbicides-properties, synthesis and control of weeds, 2012. 14: p. 247-284. https://doi.org/10.5772/32491
17. Singh, S., et al., Glyphosate uptake, translocation, resistance emergence in crops, analytical monitoring, toxicity and degradation: a review. Environmental chemistry letters, 2020. 18(3): p. 663-702.
18. Dupmeier, T., Agricultural Pesticide Threats to Animal Production and Sustainability, in Synthetic Pesticide Use in Africa2021, CRC Press. p. 129-146.
19. Vicini, J.L., et al., Glyphosate in livestock: feed residues and animal health. Journal of animal science, 2019. 97(11): p. 4509-4518.
20. Giaquinto, P.C., et al., Effects of glyphosate-based herbicide sub-lethal concentrations on fish feeding behavior. Bulletin of Environmental Contamination and Toxicology, 2017. 98(4): p. 460-464.
21. Van Bruggen, A.H., et al., Environmental and health effects of the herbicide glyphosate. Science of the total environment, 2018. 616: p. 255-268.
22. Fathi, M.A., et al., Disruption of cytochrome P450 enzymes in the liver and small intestine in chicken embryos in ovo exposed to glyphosate. Environmental science and pollution research, 2020. 27(14): p. 16865-16875. https://doi.org/10.1007/s11356-020-08269-3
23. Garg, U.K., et al., Haemato-biochemical and immuno-pathophysiological effects of chronic toxicity with synthetic pyrethroid, organophosphate and chlorinated pesticides in broiler chicks. International immunopharmacology, 2004. 4(13): p. 1709-1722.
24. Hussain, R., et al., Clinico-hematological and tissue changes induced by butachlor in male Japanese quail (Coturnix japonica). Pesticide biochemistry and physiology, 2014. 109: p. 58-63.
25. Heymann, A.-K., et al., Effects of glyphosate residues and different concentrate feed proportions in dairy cow rations on hepatic gene expression, liver histology and biochemical blood parameters. Plos one, 2021. 16(2): p. e0246679. https://doi.org/10.1371/journal.pone.0246679
26. Akçay, A., The calculation of LD50 using probit analysis, 2013, Wiley Online Library.
27. Yin, X., et al., Genotoxicity evaluation of chlorpyrifos to amphibian Chinese toad (Amphibian: Anura) by comet assay and micronucleus test. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2009. 680(1-2): p. 2-6. https://doi.org/10.1016/j.mrgentox.2009.05.018
28. Fenech, M., et al., HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2003. 534(1-2): p. 65-75.
29. Jasper, R., et al., Evaluation of biochemical, hematological and oxidative parameters in mice exposed to the herbicide glyphosate-Roundup®. Interdisciplinary toxicology, 2012. 5(3): p. 133. https://doi.org/10.2478/v10102-012-0022-5
30. Galli, F.S., et al., Overview of human health effects related to glyphosate exposure. Frontiers in toxicology, 2024. 6: p. 1474792.
31. Kronberg, M.F., A. Rossen, and E.R. Munarriz, Glyphosate-based herbicides and oxidative stress, in Toxicology2021, Elsevier. p. 79-90.
32. Rieg, C.E.H., et al., Perinatal exposure to a glyphosate pesticide formulation induces offspring liver damage. Toxicology and applied pharmacology, 2022. 454: p. 116245.
33. Ati, S., et al., Impact of Glyphosate herbicide on blood parameters and selected tissues organs of rabbits (oryctolagus cuniculus). Brazilian Journal of Animal and Environmental Research, 2024. 7(4): p. e75546-e75546.
34. Campos-Pereira, F.D., et al., Early cytotoxic and genotoxic effects of atrazine on Wistar rat liver: a morphological, immunohistochemical, biochemical, and molecular study. Ecotoxicology and environmental safety, 2012. 78: p. 170-177. https://doi.org/10.1016/j.ecoenv.2011.11.020
35. Çavaş, T. and S. Ergene‐Gözükara, Micronucleus test in fish cells: a bioassay for in situ monitoring of genotoxic pollution in the marine environment. Environmental and molecular mutagenesis, 2005. 46(1): p. 64-70. https://doi.org/10.1002/em.20130
36. Riaz Hussain, R.H., et al., Cellular and biochemical effects induced by atrazine on blood of male Japanese quail (Coturnix japonica). 2012.
37. de Campos Ventura, B., D.d.F. de Angelis, and M.A. Marin-Morales, Mutagenic and genotoxic effects of the Atrazine herbicide in Oreochromis niloticus (Perciformes, Cichlidae) detected by the micronuclei test and the comet assay. Pesticide biochemistry and physiology, 2008. 90(1): p. 42-51. https://doi.org/10.1016/j.pestbp.2007.07.009
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