Butea Monosperma's Phytochemical Properties and its Antibacterial, Antioxidant, and Hypoglycemic Effects

Zaafran Khan; Anum Zeb; Fatima Akbar Sheikh

doi:10.70749/ijbr.v3i09.2246

Authors

Zaafran Khan Department of Biotechnology, Mirpur University of Science and Technology (MUST), AJK, Pakistan.
Anum Zeb Medical College, Hubei University of Arts and Science, Xiangyang City, Hubei Province, China.
Fatima Akbar Sheikh College of Pharmacy, Niazi Medical & Dental College, Sargodha 40100, Pakistan.

DOI:

https://doi.org/10.70749/ijbr.v3i09.2246

Keywords:

Butea monosperma, phytochemicals, antibacterial activity, antioxidant activity, hypoglycemic effect, methanolic extract, traditional medicine

Abstract

The medicinal traditions of Butea monosperma (flame of the forest) are supported by its diverse phytochemical profile and multiple biological activities. This study investigates the antibacterial, antioxidant, and hypoglycemic properties of ethanol, methanol, ethyl acetate, and chloroform extracts from the leaves, flowers, bark, and seeds of the plant. Phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, sterols, and glycosides, with methanolic extracts showing the highest abundance. Antibacterial activity, assessed via the disk diffusion method, showed significant inhibition of both Gram-positive and Gram-negative bacteria, with methanolic leaf extract demonstrating the largest inhibition zones against Pseudomonas aeruginosa and Staphylococcus aureus. Antioxidant activity, evaluated using the DPPH free radical scavenging assay, was most potent in methanolic leaf and flower extracts. Furthermore, methanolic flower extract exhibited a pronounced hypoglycemic effect in alloxan-induced diabetic rabbits, reducing blood glucose by 44% over three weeks and stabilizing body weight. These findings reinforce the therapeutic potential of Butea monosperma as a natural source of antibacterial, antioxidant, and hypoglycemic agents, validating its use in traditional medicine and highlighting its promise for pharmaceutical applications.

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References

1. Dave, K., P. Darji, and F. Gandhi, Antimicrobial activity and phytochemical study of plant parts of Butea monosperma. J Drug Deliv Ther, 2019. 9(4-A): p. 344-348.

https://doi.org/10.22270/jddt.v9i4-a.3435

2. Talubmook, C. and N. Buddhakala, Antioxidant and antidiabetic activities of flower extract from Butea monosperma (Lam.) Taub. GSTF International Journal on Bioformatics & Biotechnology (JBio), 2012. 2(1): p. 7.

https://doi.org/10.5176/2251-3140_2.1.16

3. Mishra, A., S. Verma, and A.P. Mishra, A plant review: Butea monosperma (Lam.) Kuntze. Res. J. Pharm. Biol. Chem. Sci, 2012. 3(1): p. 700-714.

4. Farooq, M.U., et al., UHPLC-QTOF-MS/MS based phytochemical characterization and anti-hyperglycemic prospective of hydro-ethanolic leaf extract of Butea monosperma. Scientific Reports, 2020. 10(1): p. 3530.

https://doi.org/10.1038/s41598-020-60076-5

5. Srivastava, A., A. Sur, and K.K. Nayak, Butea monosperma: A plant of traditional and medicinal significance. NewBioWorld, 2022. 4(2): p. 8-14.

https://doi.org/10.52228/nbw-jaab.2022-4-2-2

6. Lahori, P. and S. Jain, Butea monosperma (Lam.) Taub: Review on its chemistry, morphology, ethnomedical uses, phytochemistry and pharmacological activities. Journal of Innovation and Invention in Pharmaceutical Sciences (JIIPS) Volume, 2020. 1(2): p. 26.

7. Bayyurt, N. and S. Rizvi, Impact of talent management on perceived organizational effectiveness: Service industry in Lahore, Pakistan. Research Journal of Business and Management, 2015. 2(4): p. 468-487.

https://doi.org/10.17261/pressacademia.2015414450

8. Thida, M., et al., In vitro Investigation on Antidiabetic and Antioxidant Assessments of Partially Purified Fractions of Cassia siamea and Butea monosperma: Indigenous Myanmar Medicinal Plant. Journal of Biologically Active Products from Nature, 2023. 13(3): p. 284-299.

https://doi.org/10.1080/22311866.2023.2239763

9. Ambastha, S. and L. Sharan, Review on medicinal importance of Butea monosperma Lam.(Taub). Def Life Sci J, 2023. 8: p. 83-92.

https://doi.org/10.14429/dlsj.8.18536

10. Jain, S. and P. Dubey, Butea monosperma (Lam.) Taub: Review on its chemistry, morphology, ethnomedical uses, phytochemistry and pharmacological activities. Journal of Drug Delivery & Therapeutics, 2023. 13(4).

https://doi.org/10.22270/jddt.v13i4.5782

11. Saroj, P. and N. Shah, Butea monosperma (Palash)–Its Ethnobotanical Knowledge, Phytochemical Studies, Pharmacological Aspects and Future Prospects.

12. Methaniya, D., R. Rathore, and H. Solanki, Butea monosperma: Ethanomedicinal studies and pharmacology: A review. International Association of Biologicals and Computational Digest, 2023. 2(1): p. 222-229.

https://doi.org/10.56588/iabcd.v2i1.140

13. Dhakad, G.G., et al., Review on Butea monosperma Plant and Its Medicinal Use. Research Journal of Pharmacology and Pharmacodynamics, 2023. 15(2): p. 69-72.

https://doi.org/10.52711/2321-5836.2023.00014

14. Khursheed, N., et al., Therapeutic Potential and Pharmacological Properties, Phytochemical Constituents of Butea monosperma (Lam.) Kuntze Syn. B. frondosa Roxb. Ex Willd.(Gul-i-Tesū). Hippocratic Journal of Unani Medicine, 2023. 18(1): p. 9-17.

https://doi.org/10.4103/hjum.hjum_33_25

15. Polina, S., N. Marka, and D. Manohar Rao, Preliminary screening of anti-microbial, anti-oxidant and anti-cancer potential of Butea monosperma flower extracts. Indian J Pure Appl Biosci, 2020. 8: p. 442-54.

https://doi.org/10.18782/2582-2845.8445

16. Neupane, A. and P. Aryal, Medicinal Values of Butea monosperma: A. Asian Journal of Pharmacognosy, 2022. 6(2): p. 6-13.

17. Chauhan, S.S. and P.K. Mahish, Flavonoids of the flame of forest-Butea monosperma. Research Journal of Pharmacy and Technology, 2020. 13(11): p. 5647-5653.

18. Sultana, S., Chapter-8 A Comprehensive Review on the Pharmacological Potential of Butea monosperma (Lam.). AND ITS BENEFITS, 2022. 141.

19. Dintu, K., et al., Butea monosperma (Lam.) Kuntze and its ethnobotanical significance.

20. Akbar, S., Butea monosperma (Lam.) Taub.(Fabaceae/Leguminosae) (Syns.: B. braamania DC. B. frondosa Roxb. ex Willd.; Erythrina monosperma Lam.; Phaso monosperma (Lam.) Kuntze), in Handbook of 200 Medicinal Plants: A Comprehensive Review of Their Traditional Medical Uses and Scientific Justifications. 2020, Springer. p. 471-478.

21. Ahmad, W., et al., Dhāk Butea Monosperma-A Review On Ethnobotanical And Unani Prospect As Well As Phytochemical And Pharmacological Properties. Ind. J. Unani Med, 2022. 15(2): p. 121-131.

https://doi.org/10.53390/ijum.2022.15207

22. Upadhyay, Y., Palasha, a holy plant: Its phytochemistry, biological effects, and therapeutic uses. International Journal of Green Pharmacy (IJGP), 2022. 16(4).

23. Rahman, M.M. and M.F. Hossen, The phytochemicals and pharmacological activity of five potential medicinal plants in the Fabaceae family: A review. Journal of Pharmacognosy and Phytochemistry, 2025. 14(2): p. 108-114.

https://doi.org/10.22271/phyto.2025.v14.i2b.15281

24. Mahanthesh, M., et al., Swiss ADME prediction of phytochemicals present in Butea monosperma (Lam.) Taub. Journal of Pharmacognosy and Phytochemistry, 2020. 9(3): p. 1799-1809.

25. Ahire, D.K. and K.S. Ruptake, Ethnobotanical Studies of Butea monosperma (Lam.) Kuntze in North Maharashtra, India.

26. Prasad, M., et al., Butea monosperma stem bark extract partially reverses high fat diet-induced obesity in rats. European Chemical Bulletin, 2023. 12(5): p. 4267-4273.

27. Jadhao, U.P., et al., Formulation And Evaluation Of Butea Monosperma FACE TONER. American Journal of Pharmaceutical Research, 2025. 15(06).

28. Faisal, Z., et al., Phytochemical profile and food applications of edible flowers: A comprehensive treatise. Journal of Food Processing and Preservation, 2022. 46(11): p. e17061.

https://doi.org/10.1111/jfpp.17061

29. Fakhri, S., et al., Antioxidant and anticancer potentials of edible flowers: where do we stand? Critical Reviews in Food Science and Nutrition, 2022. 62(31): p. 8589-8645.

https://doi.org/10.1080/10408398.2021.1931022

30. Dulala, R.K., et al., Phytochemical cocktail of Asanadi ganaextract in the management of diabetes. Bioinformation, 2023. 19(3): p. 299.

https://doi.org/10.6026/97320630019299

31. Prabawati, N.B., et al., Edible flowers: Antioxidant compounds and their functional properties. Horticulturae, 2021. 7(4): p. 66.

https://doi.org/10.3390/horticulturae7040066

32. Ravichandiran, K. and M. Parani, Comparative analysis of the metabolites from the rind, pulp and seeds of two varieties of bitter gourd using Gas Chromatography-Mass Spectrometry (GC-MS). Research Journal of Biotechnology Vol, 2022. 17: p. 11.

https://doi.org/10.25303/1711rjbt37043

33. Rokade, V.G., V.K. Gurjar, and S.P. Kharat, Development and evaluation of herbal formulation of ficus religiosa. 2024.