Effect of Vitamin D Replacement in Patient with Sub-clinical Hypothyroidism
DOI:
https://doi.org/10.70749/ijbr.v3i10.2373Keywords:
Endocrine System, Hypothyroidism, Pakistan, Thyroid Diseases, Vitamin D.Abstract
Background: Subclinical hypothyroidism defines elevated serum thyroid stimulating hormone with normal thyroxine in the circulation. While often symptomless, subclinical hypothyroidism can evolve towards overt disease and cause metabolic as well as cardiovascular complications. Deficiency in vitamin D is extremely common and has been implicated in defective thyroid physiology by virtue of its immunomodulatory action. It has been posited that supplementation might correct thyroidal as well as metabolic outcomes in individuals so affected. Objective: To evaluate the effect of vitamin D replacement on thyroid stimulating hormone levels in patients with subclinical hypothyroidism. Study Design: It was Quasi-experimental study. Study Duration and Setting: Conducted from June 2025 to August 2025 at PAF Hospital, Islamabad. Methodology: A total of 60 patients aged 18–60 years with subclinical hypothyroidism were included. Participants received oral vitamin D supplementation of 50,000 IU weekly for eight weeks. Serum thyroid stimulating hormone, thyroxine, and vitamin D levels were measured before and after intervention. Results: The mean age of participants was 38.5 ± 12.7 years, with female predominance (78.3%). Vitamin D supplementation significantly reduced mean thyroid stimulating hormone levels from 7.05 ± 1.25 to 3.44 ± 1.38 mIU/L (p < 0.001). Serum Vit D increased from 16.07 ± 5.02 to 37.08 ± 7.22 ng/mL (p < 0.001). Conclusion: Vitamin D replacement therapy effectively improves thyroid function in patients with subclinical hypothyroidism.
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References
1. Jasim, S., Abdi, H., Gharib, H., & Biondi, B. (2021). A clinical debate: Subclinical hypothyroidism. International Journal of Endocrinology and Metabolism, 19(3).
https://doi.org/10.5812/ijem.115948
2. Yoo, W. S., & Chung, H. K. (2021). Subclinical hypothyroidism: Prevalence, health impact, and treatment landscape. Endocrinology and Metabolism, 36(3), 500-513.
https://doi.org/10.3803/enm.2021.1066
3. Zamwar, U. M., & Muneshwar, K. N. (2023). Epidemiology, types, causes, clinical presentation, diagnosis, and treatment of hypothyroidism. Cureus.
https://doi.org/10.7759/cureus.46241
4. Li, J., Xu, Y., Sun, Z., Cai, Y., Wang, B., Zhang, M., Ban, Y., Hou, X., Hao, Y., Ouyang, Q., Wu, B., Wang, M., & Wang, W. (2021). Differential lipids in pregnant women with subclinical hypothyroidism and their correlation to the pregnancy outcomes. Scientific Reports, 11(1).
https://doi.org/10.1038/s41598-021-99252-6
5. Sharma, V. K., Niraula, A., Tuladhar, E. T., Bhattarai, A., Raut, M., Dubey, R. K., Baidya, S., & Parajuli, N. (2023). Autoimmune thyroid status in subclinical thyroid disorders in patients attending a tertiary care center in Nepal: A hospital-based cross-sectional study. BMC Endocrine Disorders, 23(1).
https://doi.org/10.1186/s12902-023-01480-6
6. Wang, J., Mei, L., Hao, Y., Xu, Y., Yang, Q., Dai, Z., Yang, Y., Wu, Z., & Ji, Y. (2024). Contemporary perspectives on the role of vitamin D in enhancing gut health and its implications for preventing and managing intestinal diseases. Nutrients, 16(14), 2352.
https://doi.org/10.3390/nu16142352
7. Athanassiou, L., Mavragani, C. P., & Koutsilieris, M. (2022). The immunomodulatory properties of vitamin D. Mediterranean Journal of Rheumatology, 33(1), 7.
https://doi.org/10.31138/mjr.33.1.7
8. Herdea, A., Marie, H., Ionescu, A., Sandu, D., Pribeagu, S., & Ulici, A. (2024). Vitamin D deficiency—A public health issue in children. Children, 11(9), 1061.
https://doi.org/10.3390/children11091061
9. Czarnywojtek, A., Florek, E., Pietrończyk, K., Sawicka-Gutaj, N., Ruchała, M., Ronen, O., Nixon, I. J., Shaha, A. R., Rodrigo, J. P., Tufano, R. P., Zafereo, M., Randolph, G. W., & Ferlito, A. (2023). The role of vitamin D in autoimmune thyroid diseases: A narrative review. Journal of Clinical Medicine, 12(4), 1452.
https://doi.org/10.3390/jcm12041452
10. Pingitore, A., Mastorci, F., Berti, S., Sabatino, L., Palmieri, C., Iervasi, G., & Vassalle, C. (2021). Hypovitaminosis D and low T3 syndrome: A link for therapeutic challenges in patients with acute myocardial infarction. Journal of Clinical Medicine, 10(22), 5267.
https://doi.org/10.3390/jcm10225267
11. Peng, B., Wang, W., Gu, Q., Wang, P., Teng, W., & Shan, Z. (2024). Effects of different supplements on Hashimoto’s thyroiditis: A systematic review and network meta-analysis. Frontiers in Endocrinology, 15.
https://doi.org/10.3389/fendo.2024.1445878
12. Pezeshki, B., Ahmadi, A., & Karimi, A. (2020). The effect of vitamin D replacement on patient with subclinical hypothyroidism: A pilot randomized clinical trial. Galen Medical Journal, 9, e1592.
https://doi.org/10.31661/gmj.v9i0.1592
13. Safari, S., Rafraf, M., Malekian, M., Molani-Gol, R., Asghari-Jafarabadi, M., & Mobasseri, M. (2023). Effects of vitamin D supplementation on metabolic parameters, serum irisin and obesity values in women with subclinical hypothyroidism: A double-blind randomized controlled trial. Frontiers in Endocrinology, 14.
https://doi.org/10.3389/fendo.2023.1306470
14. Rathi Roopavathy, J., & Chandramouleeswari, K. (2019). A study on serum vitamin D levels among subclinical hypothyroid adult females from a tertiary health care institute – case control study. Int J Curr Med Appl Sci, 22(3), 30-33.
https://www.ijcmaas.com/images/archieve/IJCMAAS_MAY_2019_VOL22_ISS3_04PDF.pdf
15. Amer, A., H., Chaudhari, K., S., Makadia., M. G., G. & Patel, H. (2023). Study of vitamin D3 level in non-diabetic patients with subclinical hypothyroidism. Int J Pharm Clin Res, 15(6), 1048-1054.
https://impactfactor.org/PDF/IJPCR/15/IJPCR,Vol15,Issue6,Article138.pdf
16. Elamawy, M., & Gouda Ameen, S. (2021). Vitamin D deficiency in subclinical hypothyroid dysfunction patients: A case control study. Benha Medical Journal, 38(1), 137-145.
https://doi.org/10.21608/bmfj.2021.143528
17. Copari-Vargas, E., Copari-Vargas, T. L., Domínguez-Valdez, L. F., Copari-Vargas, L. E., & Copari-Jimenez, E. (2025). Vitamin D status and its association with disease severity in Hashimoto’s thyroiditis. Cureus.
https://doi.org/10.7759/cureus.83419
18. Fang, Y., Wen, X., You, H., Huang, Y., Qu, S., Wang, X., & Bu, L. (2025). Decreased vitamin D increase the risk for subclinical hypothyroidism in individuals with T2DM: A cross-sectional study. Frontiers in Nutrition, 12.
https://doi.org/10.3389/fnut.2025.1509465
19. Dülger, Ö., OĞUL, Z., & Dinmez, S. (2025). The correlation between vitamin D levels and thyroid functions in early pregnancy. Eastern Journal Of Medicine, 30(1), 29-36.
https://doi.org/10.5505/ejm.2025.47750
20. Wu, L., Liang, B., Lin, R., Fu, Z., Huang, H., & Zhou, J. (2025). Correlation analysis of thyroid function and vitamin D levels in patients with type 2 diabetes. Frontiers in Endocrinology, 16.
https://doi.org/10.3389/fendo.2025.1650525
21. Appunni, S., Rubens, M., Ramamoorthy, V., Saxena, A., Tonse, R., Veledar, E., & McGranaghan, P. (2021). Association between vitamin D deficiency and hypothyroidism: Results from the national health and nutrition examination survey (NHANES) 2007–2012. BMC Endocrine Disorders, 21(1).
https://doi.org/10.1186/s12902-021-00897-1
22. Jebur, A. S., Saleh, B. O., & Al Azzawi, O. F. (2025). Vitamin D and the pathogenesis of primary hypothyroidism. Journal of the Faculty of Medicine Baghdad.
https://doi.org/10.32007/jfacmedbaghdad3123
23. Rajendiran, V., Gunasekaran, D., Venkatesh, S., Dhayalan, I., & Srinivasaraghavan, R. (2018). Prevalence of sub-clinical vitamin-D deficiency and hypothyroidism in children aged 18- 36 months with open anterior fontanelle: A cross-sectional study. International Journal of Contemporary Pediatrics, 5(2), 350.
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