Effect of Sono-Ohmic Pasteurizer on Microbial, Physicochemical and Sensorial Properties of Milk

Authors

  • Hafiz Danish Butt Department of Food Engineering, University of Agriculture, Faisalabad, Punjab, Pakistan
  • Syeda Noor-ul-Ain Naqvi National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Punjab, Pakistan.
  • Muqadas Iqbal National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Punjab, Pakistan. 3Government College Women University, Faisalabad, Punjab, Pakistan
  • Hira Shair National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Punjab, Pakistan.Wheat Research Institute, Ayub Agricultural Research Institute, Faisalabad, Punjab, Pakistan
  • Abdul Rauf Rajput Institute of Food Science and Technology, Sindh Agriculture University, Tandojam, Sindh, Pakistan.
  • Muhammad Kashif Iqbal Khan Department of Food Engineering, University of Agriculture, Faisalabad, Punjab, Pakistan.National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Punjab, Pakistan.
  • Aman Arif Department of Nutritional Sciences, Government College University, Faisalabad, Punjab, Pakistan.

DOI:

https://doi.org/10.70749/ijbr.v3i12.2716

Keywords:

Milk, Pasteurization, Ohmic, Microbes, Temperature

Abstract

Milk, is a perfect food that is nutritious, and essential part of the human diet. It is perishable, and consumers want to use it fresh. It is being processed to increase the shelf life for later use. Traditional thermal methods of pasteurizing and sterilizing foods are based on heat transfer. These methods cause color loss , flavor, and nutrients due to loss of protein, and volatile compounds. Milk samples were treated at different temperatures for different time duration and the best combination of both was observed. For this purpose, milk was treated at different temperatures (50˚C, 60˚C, 63˚C) for 10, 15, and 20 minutes respectively. The study showed pH values were 6.36 to 6.67, and titratable acidity of 0.15 to 0.11, indicating that the sono-ohmic pasteurization technique slightly affected these parameters. The value of brix was 5.8 to 9.2, indicating a significant effect. However, the coliform had significantly decreased from 12.16 to 11.47 log10 CFU/mL, and yeast mold value from 11.99 to 11.66 log10 CFU/mL, exhibited significant impacts on milk quality. The total plate count, coliform bacteria, mold, and yeast have been linked to good milk quality and decreased risk of  consumers health illnesses. The best treatment of milk was treated at 63˚C for 20 minutes and the storage study was evaluated at refrigeration temperature. The storage study showed pH values, brix, titratable acidity, coliform and yeast mold, indicating that the sono-ohmic pasteurization technique had a significant effect (P<0.05) on these parameters. Increased value of TPC, coliform bacteria, mold, and yeast had caused the deterioration of milk which is not good for health. Results showed that sono-ohmicpasteurization was an efficient process.

Downloads

Download data is not yet available.

References

Aadil, R. M., Khalil, A. A., Rehman, A., Khalid, A., Inam‐ur‐Raheem, M., Karim, A., Gill, A. A., Abid, M., & Afraz, M. T. (2020). Assessing the impact of ultra‐sonication and thermo‐ultrasound on antioxidant indices and polyphenolic profile of Apple‐grape juice blend. Journal of Food Processing and Preservation, 44(5).

https://doi.org/10.1111/jfpp.14406

Achir, N., Dhuique-Mayer, C., Hadjal, T., Madani, K., Pain, J., & Dornier, M. (2016). Pasteurization of citrus juices with ohmic heating to preserve the carotenoid profile. Innovative Food Science & Emerging Technologies, 33, 397-404.

https://doi.org/10.1016/j.ifset.2015.11.002

Afzali, S., Edalatian Dovom, M. R., Habibi Najafi, M. B., & Mazaheri Tehrani, M. (2020). Determination of the anti-yeast activity of lactobacillus spp. isolated from traditional Iranian cheeses in vitro and in yogurt drink (Doogh). Scientific Reports, 10(1).

https://doi.org/10.1038/s41598-020-63142-0

AOAC (Association of Official Analytical Chemists). 2019. The Official Methods of Analysis of Association of Official Analytical Chemists International, 21st Ed. AOAC International, Arlington, U.S.A.

Awasti, N., & Anand, S. (2020). The role of yeast and molds in dairy industry: An update. Dairy Processing: Advanced Research to Applications, 243-262.

https://doi.org/10.1007/978-981-15-2608-4_12

Beldie, A. A., & Moraru, C. I. (2021). Forward osmosis concentration of milk: Product quality and processing considerations. Journal of Dairy Science, 104(7), 7522-7533.

https://doi.org/10.3168/jds.2020-20019

Bevilacqua, A., Petruzzi, L., Perricone, M., Speranza, B., Campaniello, D., Sinigaglia, M., & Corbo, M. R. (2017). Nonthermal technologies for fruit and vegetable juices and beverages: Overview and advances. Comprehensive Reviews in Food Science and Food Safety, 17(1), 2-62.

https://doi.org/10.1111/1541-4337.12299

Bezie, A. (2019). The effect of different heat treatment on the nutritional value of milk and milk products and shelf-life of milk products. A review. Journal of Dairy & Veterinary Sciences, 11(5).

https://doi.org/10.19080/jdvs.2019.11.555822

Bhattacharjee, A., & Chakraborty, S. (2022). Design of a batch ohmic heater and evaluating the influence of different treatment conditions on quality attributes of kinnow (Citrus nobilis × citrus deliciosa) juice. Innovative Food Science & Emerging Technologies, 82, 103186.

https://doi.org/10.1016/j.ifset.2022.103186

Cappato, L., Ferreira, M., Guimaraes, J., Portela, J., Costa, A., Freitas, M., Cunha, R., Oliveira, C., Mercali, G., Marzack, L., & Cruz, A. (2017). Ohmic heating in dairy processing: Relevant aspects for safety and quality. Trends in Food Science & Technology, 62, 104-112.

https://doi.org/10.1016/j.tifs.2017.01.010

Carillo, P., Ciarmiello, L. F., Woodrow, P., Corrado, G., Chiaiese, P., & Rouphael, Y. (2020). Enhancing sustainability by improving plant salt tolerance through macro- and micro-algal biostimulants. Biology, 9(9), 253.

https://doi.org/10.3390/biology9090253

Cheng, N., Barbano, D. M., & Drake, M. A. (2018). Hunter versus CIE color measurement systems for analysis of milk-based beverages. Journal of Dairy Science, 101(6), 4891-4905.

https://doi.org/10.3168/jds.2017-14197

CHUDY, S., BILSKA, A., KOWALSKI, R., & TEICHERT, J. (2020). Colour of milk and milk products in CIE L*a*b* space. Medycyna Weterynaryjna, 76(01), 6327-2020.

https://doi.org/10.21521/mw.6327

Ding, R., Yang, S., Geng, L., Liu, Y., He, B., Liu, L., Yue, X., Wu, R., & Wu, J. (2023). Characterization of the core microflora and nutrient composition in packaged pasteurized milk products during storage. Food Science and Human Wellness, 12(4), 1279-1286.

https://doi.org/10.1016/j.fshw.2022.10.010

Eazhumalai, G., Ranjitha Gracy, T. K., Mishra, A., & Annapure, U. S. (2021). Atmospheric pressure nonthermal pin to plate plasma system for the microbial decontamination of oat milk. Journal of Food Processing and Preservation, 46(10).

https://doi.org/10.1111/jfpp.16181

Ferrario, M., Alzamora, S. M., & Guerrero, S. (2015). Study of the inactivation of spoilage microorganisms in Apple juice by pulsed light and ultrasound. Food Microbiology, 46, 635-642.

https://doi.org/10.1016/j.fm.2014.06.017

Foroutan, A., Guo, A. C., Vazquez-Fresno, R., Lipfert, M., Zhang, L., Zheng, J., Badran, H., Budinski, Z., Mandal, R., Ametaj, B. N., & Wishart, D. S. (2019). Chemical composition of commercial cow’s milk. Journal of Agricultural and Food Chemistry, 67(17), 4897-4914.

https://doi.org/10.1021/acs.jafc.9b00204

Gavahian, M., S. Sastry, R. Farhoosh and A. Farahnaky. (2019). Ohmic heating as a promising technique for extraction of herbal essential oils: Understanding mechanisms, recent findings, and associated challenges. Advances in Food and Nutrition Research 91:227-273.

https://doi.org/10.1016/bs.afnr.2019.09.001

Goullieux, A., & Pain, J. P. (2014). Ohmic heating. In Emerging technologies for food processing (pp. 399-426). Academic Press.

Hashemi, S. M., Gholamhosseinpour, A., & Niakousari, M. (2019). Application of microwave and ohmic heating for pasteurization of cantaloupe juice: Microbial inactivation and chemical properties. Journal of the Science of Food and Agriculture, 99(9), 4276-4286.

https://doi.org/10.1002/jsfa.9660

Ahmed Hass, N. B., Mohamed Ab, M. O., & Mohamed No, A. A. (2009). Microbiological quality of heat-treated milk during storage. Pakistan Journal of Nutrition, 8(12), 1845-1848.

https://doi.org/10.3923/pjn.2009.1845.1848

Jadhav, H. B., Raina, I., Gogate, P. R., Annapure, U. S., & Casanova, F. (2023). Sonication as a promising technology for the extraction of Triacylglycerols from fruit seeds—A review. Food and Bioprocess Technology, 16(8), 1625-1651.

https://doi.org/10.1007/s11947-022-02987-x

Jaeger, H., Roth, A., Toepfl, S., Holzhauser, T., Engel, K., Knorr, D., Vogel, R. F., Bandick, N., Kulling, S., Heinz, V., & Steinberg, P. (2016). Opinion on the use of ohmic heating for the treatment of foods. Trends in Food Science & Technology, 55, 84-97.

https://doi.org/10.1016/j.tifs.2016.07.007

Knirsch, M. C., Alves dos Santos, C., Martins de Oliveira Soares Vicente, A. A., & Vessoni Penna, T. C. (2010). Ohmic heating – a review. Trends in Food Science & Technology, 21(9), 436-441.

https://doi.org/10.1016/j.tifs.2010.06.003

Kumar, V., & Longhurst, P. (2018). Recycling of food waste into chemical building blocks. Current Opinion in Green and Sustainable Chemistry, 13, 118-122.

https://doi.org/10.1016/j.cogsc.2018.05.012

Lascorz, D., Torella, E., Lyng, J. G., & Arroyo, C. (2016). The potential of ohmic heating as an alternative to steam for heat processing shrimps. Innovative Food Science & Emerging Technologies, 37, 329-335.

https://doi.org/10.1016/j.ifset.2016.06.014

Lemos, Á. T., Lopes-da-Silva, J. A., Delgadillo, I., & Saraiva, J. A. (2023). Preservation of high pressure pasteurised milk by hyperbaric storage at room temperature versus refrigeration – Effect on natural microbiota and physicochemical properties. Food Chemistry Advances, 2, 100241.

https://doi.org/10.1016/j.focha.2023.100241

Lim, S., Benner, L. C., & Clark, S. (2019). Neither thermosonication nor cold sonication is better than pasteurization for milk shelf life. Journal of Dairy Science, 102(5), 3965-3977.

https://doi.org/10.3168/jds.2018-15347

Manzoor, F., Wei, L., Asif, M., Haq, M. Z., & Rehman, H. U. (2019). The contribution of sustainable tourism to economic growth and employment in Pakistan. International Journal of Environmental Research and Public Health, 16(19), 3785.

https://doi.org/10.3390/ijerph16193785

Manzoor, M. F., Siddique, R., Hussain, A., Ahmad, N., Rehman, A., Siddeeg, A., Alfarga, A., Alshammari, G. M., & Yahya, M. A. (2021). Thermosonication effect on bioactive compounds, enzymes activity, particle size, microbial load, and sensory properties of almond (Prunus dulcis) milk. Ultrasonics Sonochemistry, 78, 105705.

https://doi.org/10.1016/j.ultsonch.2021.105705

Marouf, A., & Sara, I. E. (2018). Monitoring pH during pasteurization of raw cow’s milk using Nd: YAG laser. International Journal of Advanced Research in Physical Science (IJARPS), 4(12), 1-4.

Martin, N. H., Trmčić, A., Hsieh, T., Boor, K. J., & Wiedmann, M. (2016). The evolving role of coliforms as indicators of unhygienic processing conditions in dairy foods. Frontiers in Microbiology, 7.

https://doi.org/10.3389/fmicb.2016.01549

Mbye, M., Sobti, B., Al Nuami, M. K., Al Shamsi, Y., Al Khateri, L., Al Saedi, R., Saeed, M., Ramachandran, T., Hamed, F., & Kamal-Eldin, A. (2020). Physicochemical properties, sensory quality, and coagulation behavior of camel versus bovine milk soft unripened cheeses. NFS Journal, 20, 28-36.

https://doi.org/10.1016/j.nfs.2020.06.003

Mennane, Z., Ouhssine, M., Khedid, K., & Elyachioui, M. (2007). Hygienic quality of raw cow’s milk feeding from domestic waste in two regions in Morocco. International journal of agriculture and biology, 9(1), 46-48.

Milovanovic, B., Tomovic, V., Djekic, I., Miocinovic, J., Solowiej, B. G., Lorenzo, J. M., Barba, F. J., & Tomasevic, I. (2021). Colour assessment of milk and milk products using computer vision system and colorimeter. International Dairy Journal, 120, 105084.

https://doi.org/10.1016/j.idairyj.2021.105084

Mohideen, F. W., Solval, K. M., Li, J., Zhang, J., Chouljenko, A., Chotiko, A., Prudente, A. D., Bankston, J. D., & Sathivel, S. (2015). Effect of continuous ultra-sonication on microbial counts and physico-chemical properties of blueberry (Vaccinium corymbosum) juice. LWT - Food Science and Technology, 60(1), 563-570.

https://doi.org/10.1016/j.lwt.2014.07.047

Montgomery, D.C. (2017). Design and analysis of experiments, John wiley & sons. Inc. Hoboken, NJ, USA.

Olusola, J. A., Akintan, O. B., Erhenhi, H. A., & Osanyinlusi, O. O. (2021). Heavy metals and health risks associated with consumption of herbal plants sold in a major urban market in Southwest, Nigeria. Journal of Health and Pollution, 11(31).

https://doi.org/10.5696/2156-9614-11.31.210915

On-Nom, N., Grandison, A., & Lewis, M. (2010). Measurement of Ionic calcium, pH, and soluble divalent cations in milk at high temperature. Journal of Dairy Science, 93(2), 515-523.

https://doi.org/10.3168/jds.2009-2634

Pegu, K., & Arya, S. S. (2023). Non-thermal processing of milk: Principles, mechanisms and effect on milk components. Journal of Agriculture and Food Research, 14, 100730.

https://doi.org/10.1016/j.jafr.2023.100730

Pham, Q., Patel, P., Baban, B., Yu, J., & Bhatia, J. (2020). Factors affecting the composition of expressed fresh human milk. Breastfeeding Medicine, 15(9), 551-558.

https://doi.org/10.1089/bfm.2020.0195

Priyadarshini, A., Rayaguru, K., & Nayak, P. K. (2022). Effect of ohmic heating and thermo-sonication on the physio-chemical, antioxidant, microbial and sensory properties of mango juice. The Indian Journal of Nutrition and Dietetics, 275-284.

https://doi.org/10.21048/ijnd.2022.59.3.29331

Rincón Rueda, M. A., Ruiz Berrio, H. D., Molano Díaz, J. M., Alvarez Herrera, J. G., & Pinto Acero, Y. L. (2021). Postharvest characterization of seven arracacha cultivars (Arracacia xanthorrhiza Bancroft). Revista Facultad Nacional de Agronomía Medellín, 74(3), 9745-9756.

https://doi.org/10.15446/rfnam.v74n3.92658

Rocha, R. S., Silva, R., Ramos, G. L., Cabral, L. A., Pimentel, T. C., Campelo, P. H., Blumer Zacarchenco, P., Freitas, M. Q., Esmerino, E., Silva, M. C., & Cruz, A. G. (2022). Ohmic heating treatment in high-protein vanilla flavored milk: Quality, processing factors, and biological activity. Food Research International, 161, 111827.

https://doi.org/10.1016/j.foodres.2022.111827

Safari, S., & Varaminian, F. (2019). Study the kinetics and thermodynamics conditions for CO2 hydrate formation in orange juice concentration. Innovative Food Science & Emerging Technologies, 57, 102155.

https://doi.org/10.1016/j.ifset.2019.04.008

Sarkar, S. (2015). Microbiological considerations: Pasteurized milk. International Journal of Dairy Science, 10(5), 206-218.

https://doi.org/10.3923/ijds.2015.206.218

Sirichan, T., Kijpatanasilp, I., Asadatorn, N., & Assatarakul, K. (2022). Optimization of ultrasound extraction of functional compound from makiang seed by response surface methodology and antimicrobial activity of optimized extract with its application in orange juice. Ultrasonics Sonochemistry, 83, 105916.

https://doi.org/10.1016/j.ultsonch.2022.105916

SUN, H., KAWAMURA, S., HIMOTO, J., ITOH, K., WADA, T., & KIMURA, T. (2008). Effects of ohmic heating on microbial counts and Denaturation of proteins in milk. Food Science and Technology Research, 14(2), 117-123.

https://doi.org/10.3136/fstr.14.117

Toffanin, V., De Marchi, M., Lopez-Villalobos, N., & Cassandro, M. (2015). Effectiveness of mid-infrared spectroscopy for prediction of the contents of calcium and phosphorus, and titratable acidity of milk and their relationship with milk quality and coagulation properties. International Dairy Journal, 41, 68-73.

https://doi.org/10.1016/j.idairyj.2014.10.002

Tripathy, S., Reddy, M. S., Vanjari, S. R., Jana, S., & Singh, S. G. (2018). A step towards miniaturized milk adulteration detection system: Smartphone-based accurate pH sensing using Electrospun Halochromic nanofibers. Food Analytical Methods, 12(2), 612-624.

https://doi.org/10.1007/s12161-018-1391-y

Varghese, F., Bukhari, A. B., Malhotra, R., & De, A. (2014). IHC profiler: An open source Plugin for the quantitative evaluation and automated scoring of Immunohistochemistry images of human tissue samples. PLoS ONE, 9(5), e96801.

https://doi.org/10.1371/journal.pone.0096801

Vroegindewey, R., Richardson, R. B., Chung, K., Theriault, V., & Ortega, D. L. (2021). Competitive advantage and processor demand for local and imported food ingredients: Analysis from an urban dairy market in West Africa. Journal of Agribusiness in Developing and Emerging Economies, 11(2), 140-159.

https://doi.org/10.1108/jadee-06-2020-0133

Wibowo, S., Grauwet, T., Santiago, J. S., Tomic, J., Vervoort, L., Hendrickx, M., & Van Loey, A. (2015). Quality changes of pasteurised orange juice during storage: A kinetic study of specific parameters and their relation to colour instability. Food Chemistry, 187, 140-151.

https://doi.org/10.1016/j.foodchem.2015.03.131

Willett, W. C., & Ludwig, D. S. (2020). Milk and health. New England Journal of Medicine, 382(7), 644-654.

https://doi.org/10.1056/nejmra1903547

Zia, M. A., Yasmin, H., Shair, F., Jabeen, Z., Mumtaz, S., Hayat, Z., Shah, S. Z., Afghan, S., Hafeez, F. Y., & Hassan, M. N. (2018). Glucanolytic Rhizobacteria produce Antifungal metabolites and elicit ROS scavenging system in sugarcane. Sugar Tech, 21(2), 244-255.

https://doi.org/10.1007/s12355-018-0654-7

Ziyaina, M., Govindan, B. N., Rasco, B., Coffey, T., & Sablani, S. S. (2018). Monitoring shelf life of pasteurized whole milk under refrigerated storage conditions: Predictive models for quality loss. Journal of Food Science, 83(2), 409-418.

https://doi.org/10.1111/1750-3841.13981

ZOU, Y., & HOU, X. (2017). Sonication enhances quality and antioxidant activity of blueberry juice. Food Science and Technology, 37(4), 599-603.

https://doi.org/10.1590/1678-457x.27816

Downloads

Published

2025-12-30

Issue

Section

Original Article

How to Cite

Butt, H. D., Naqvi, S. N.- ul-A., Iqbal, M., Shair, H., Rajput, A. R., Iqbal Khan, M. K., & Arif, A. (2025). Effect of Sono-Ohmic Pasteurizer on Microbial, Physicochemical and Sensorial Properties of Milk. Indus Journal of Bioscience Research, 3(12), 46-58. https://doi.org/10.70749/ijbr.v3i12.2716