Phytochemical Analysis of Psidium Guajava Leaf Extracts

Mahnoor Baloch; Zarlish; Samra Soomro; Saher Ujjan

doi:10.70749/ijbr.v2i02.353

Authors

Mahnoor Baloch Department of Pharmacognosy, Faculty of Pharmacy, University of Sindh, Jamshoro, Sindh, Pakistan.
Zarlish Department of Pharmacognosy, Faculty of Pharmacy, University of Sindh, Jamshoro, Sindh, Pakistan.
Samra Soomro Department of Pharmacology, Faculty of Pharmacy, University of Sindh, Jamshoro, Sindh, Pakistan.
Saher Ujjan Department of Pharmacology, Faculty of Pharmacy, University of Sindh, Jamshoro, Sindh, Pakistan.

DOI:

https://doi.org/10.70749/ijbr.v2i02.353

Keywords:

Psidium Guajava, Phytochemical Analysis, Guava Leaf Extract, Polar Solvents, Traditional Medicine, Alkaloids, Flavonoids, Tannins, Saponins, Bioactive Compounds

Abstract

Background: Psidium guajava (guava), a medicinal plant from the Myrtaceae family, has been widely used in traditional medicine for its anti-inflammatory, antimicrobial, and antioxidant properties. The plant is known for its rich phytochemical composition, which supports its therapeutic applications. Objective: This study aimed to analyze the phytochemical profile of Psidium guajava leaves using different solvent extracts to identify bioactive compounds with potential pharmacological benefits. Methods: Guava leaves were collected, shade-dried for 35 days, and ground into a coarse powder. Sequential solvent extractions were performed using ether, chloroform, ethanol, water, and hydroalcoholic solvents. Standard phytochemical tests were applied to detect alkaloids, flavonoids, tannins, saponins, sterols, carbohydrates, and other bioactive compounds. Quantitative data were statistically analyzed using SPSS (version 26). Results: Ethanolic and hydroalcoholic extracts exhibited the highest concentrations of phytochemicals. Alkaloids were strongly present in the ethanolic extract (Dragendorff’s reagent: +++, Hager’s reagent: ++). Tannins showed the highest presence in ethanolic, aqueous, and hydroalcoholic extracts (+++). Flavonoids were consistently detected in polar extracts (++). Carbohydrates were significantly abundant in ethanol, aqueous, and hydroalcoholic extracts (++). Nonpolar extracts (ether, chloroform) yielded minimal phytochemicals. Conclusion: The study confirmed the presence of significant bioactive compounds, particularly in polar extracts, validating the pharmacological potential of guava leaves and their relevance in drug development.

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References

Kumar, M., Saurabh, V., Tomar, M., Hasan, M., Changan, S., Sasi, M., Maheshwari, C., Prajapati, U., Singh, S., Prajapat, R. K., Dhumal, S., Punia, S., Amarowicz, R., & Mekhemar, M. (2021). Mango (Mangifera indica L.) leaves: Nutritional composition, phytochemical profile, and health-promoting Bioactivities. Antioxidants, 10(2), 299. https://doi.org/10.3390/antiox10020299

Farag, R. S., Abdel-Latif, M. S., Abd El Baky, H. H., & Tawfeek, L. S. (2020). Phytochemical screening and antioxidant activity of some medicinal plants’ crude juices. Biotechnology Reports, 28, e00536. https://doi.org/10.1016/j.btre.2020.e00536

Jassal, K., & Kaushal, S. (2019). Phytochemical and antioxidant screening of guava (Psidium guajava) leaf essential oil. Agricultural Research Journal, 56(3), 528. https://doi.org/10.5958/2395-146x.2019.00082.6

Biswas, S., Talukdar, P., & Talapatra, S. N. (2019). Presence of phytochemicals in fruits and leaves of guava (Psidium guajava Linn.) for cancer prevention: A mini review. Journal of Drug Delivery and Therapeutics, 9(4-s), 726-729. https://doi.org/10.22270/jddt.v9i4-s.3290

Palombo, E. A. (2006). Phytochemicals from traditional medicinal plants used in the treatment of diarrhoea: Modes of action and effects on intestinal function. Phytotherapy Research, 20(9), 717-724. https://doi.org/10.1002/ptr.1907

Ullah, F., Ayaz, M., Sadiq, A., Ullah, F., Hussain, I., Shahid, M., Yessimbekov, Z., Adhikari-Devkota, A., & Devkota, H. P. (2020). Potential role of plant extracts and phytochemicals against foodborne pathogens. Applied Sciences, 10(13), 4597. https://doi.org/10.3390/app10134597

Das, M., & Goswami, S. (2019). Antifungal and antibacterial property of guava (Psidium guajava) leaf extract: Role of phytochemicals. Int. J. Health Sci. Res, 9(2), 39-45.

Dewi, P. S., Sutjiatmo, A. B., & Nurdiansyah, A. (2013). Antidiarrheal activity of water extracts of guava leaves (Psidium guajava L.) and water extracts of green tea leaves (Camellia sinensis L.) combination in Swiss Webster mice. Acta Pharmaceutica Indonesia, 38(2), 67-70. https://doi.org/10.5614/api.v38i2.5213

Offor, C. E. (2015). Phytochemical and proximate analyses of Psidium guajava leaves. J Res Pharm Sci, 2(6), 05-07.

Emmanuel, A., Kubmarawa, D., Sara, G. Y., & Wahu, A. (2019). Phytochemical screening, antioxidant and antimicrobial activities of the essential oils and ethanol extract of Psidium guajava leaf. Asian Journal of Physical and Chemical Sciences, 1-8. https://doi.org/10.9734/ajopacs/2019/v7i430102

Kamal, A., & Khan, M. M. R. (2014). Phytochemical evaluation of some medicinal plants. Ind J Plant Sci, 3(4), 5-8. https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=f6c4ee1de08aac52fc77f815806a347c95f3d3c8

Metwally, A. M., Omar, A. A., Harraz, F. M., & El Sohafy, S. M. (2010). Phytochemical investigation and antimicrobial activity of Psidium guajava L. leaves. Pharmacognosy Magazine, 6(23), 212–218. https://doi.org/10.4103/0973-1296.66939

Barradas, Y. D., Borsoi, F. T., Dacoreggio, M. V., Moroni, L. S., Silva Rosa Bonadiman, B. D., Marafon, F., Giacobbo, C. L., Bagatini, M. D., & Kempka, A. P. (2022). Phytochemical profiling, antidiabetic, antitumoral and cytotoxic potential of Psidium cattleianum Afzel. ex Sabine leaves of red variety. Natural Product Research, 37(4), 608-612. https://doi.org/10.1080/14786419.2022.2065484

Saber, F. R., Abdelbary, G. A., Salama, M. M., Saleh, D. O., Fathy, M. M., & Soliman, F. M. (2018). Uplc/Qtof/Ms profiling of two Psidium species and the in-vivo hepatoprotective activity of their nano-formulated liposomes. Food Research International, 105, 1029-1038. https://doi.org/10.1016/j.foodres.2017.12.042

Joseph*, L., George, M., Singh, G., & Mathews, P. (2016). Phytochemical investigation on various parts of Psidium guajava. Annals of Plant Sciences, 5(02), 1265. https://doi.org/10.21746/aps.2016.02.001

Kumar, M., Tomar, M., Amarowicz, R., Saurabh, V., Nair, M. S., Maheshwari, C., Sasi, M., Prajapati, U., Hasan, M., Singh, S., Changan, S., Prajapat, R. K., Berwal, M. K., & Satankar, V. (2021). Guava (Psidium guajava L.) leaves: Nutritional composition, phytochemical profile, and health-promoting Bioactivities. Foods, 10(4), 752. https://doi.org/10.3390/foods10040752

Uboh, F. (2010). Effect of aqueous extract of Psidium Guajava leaves on liver enzymes, histological integrity and hematological indices in rats. Gastroenterology Research. https://doi.org/10.4021/gr2010.02.174w

Wadood, A. (2013). Phytochemical analysis of medicinal plants occurring in local area of Mardan. Biochemistry & Analytical Biochemistry, 02(04). https://doi.org/10.4172/2161-1009.1000144

Fu, H., Luo, Y., & Zhang, D. (2009). Studies on chemical constituents of leaves of Psidium guajava. China Journal of Chinese Materia Medica, 577-579.

Periyanayagam, K., Gopalakrishnan, S., & Karthikeyan, V. (2013). Evaluation of Pharmacognostical and Phytochemical properties of the Leaves of Psidium guajava Linn-Chittidhar variety. Innov. J. Health Sci, 1(3), 10-13.

Okunrobo, L. O., Imafidon, K. E., & Alabi, A. A. (2010). Phytochemical, proximate and metal content analysis of the leaves of Psidium guajava Linn (Myrtaceae). International Journal of Health Research, 3(4), 217-221.

Thenmozhi, S., & Rajan, S. (2015). GC-MS analysis of bioactive compounds in Psidium guajava leaves. Journal of pharmacognosy and phytochemistry, 3(5), 162-166.

Arya, V., Thakur, N., & Kashyap, C. P. (2012). Preliminary phytochemical analysis of the extracts of Psidium leaves. Journal of Pharmacognosy and Phytochemistry, 1(1), 01-05.