Isolation and Characterization of Plant Growth-Promoting Rhizobacteria Associated with Okra Cultivation in the Haripur

Shamsa Kanwal; Zahidullah; Zounaira; Kainat Fatima; Mehwish Shah; Haleema Sadia; Saud Aziz; Sayab Khan; Abdullah Jan; Muhammad Hassan Raza

doi:10.70749/ijbr.v3i1.537

Authors

Shamsa Kanwal Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad, KP, Pakistan.
Zahidullah Department of Microbiology, Hazara University, Mansehra, KP, Pakistan.
Zounaira Department of Microbiology, Hazara University, Mansehra, KP, Pakistan.
Kainat Fatima Department of Microbiology, University of Haripur, KP, Pakistan.
Mehwish Shah Department of Microbiology, Hazara University, Mansehra, KP, Pakistan.
Haleema Sadia Department of Chemistry, Abbottabad University of Science and Technology, Abbottabad, KP, Pakistan.
Saud Aziz Department of Microbiology, Hazara University, Mansehra, KP, Pakistan.
Sayab Khan Department of Microbiology, Hazara University, Mansehra, KP, Pakistan.
Abdullah Jan University of Wah, Wah Cantt, Pakistan.
Muhammad Hassan Raza University of Lahore, Islamabad Campus, Pakistan.

DOI:

https://doi.org/10.70749/ijbr.v3i1.537

Keywords:

Rhizobacteria, Plant Growth-promoting Bacteria, Rhizosphere, Microbial Diversity, Okra, Soil Microbiology

Abstract

Organic farming represents an ecologically sound and economically viable agricultural practice that enhances soil fertility and quality. However, as more complex agricultural techniques have been developed, organic farming has become increasingly limited worldwide. Culture-dependent plant growth-promoting bacterial isolates were isolated from the bulk and the rhizosphere. This study uses bacterial isolates from the Haripur region of KPK, Pakistan, as a plant growth promoter and alternative to chemical fertilizers due to the increasing environmental contamination caused by the deterioration of soil health and the use of chemical fertilizers to increase crop yield. In the region of Haripur, eleven bacteria were discovered, of which two, PGPR-2 and PGPR-8, were especially effective at promoting plant development by producing ammonia, indole, amylase, catalase, nitrate reduction, phosphate solubilization, and biological nitrogen fixation. Further investigation was conducted into the biochemical and extracellular enzymatic activities of PGPR-2 and PGPR-8. Using the Roll towel method, the germination rate, root length, and branch length of prospective vegetable isolates were measured.

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