Evaluating the Antibacterial Efficacy of Withania somnifera (Ashwagandha) against Various Bacterial Strains

Waseem Sajjad; Fahim Ullah; Rizwan Ullah; Zia Ullah; Mudassir Ahmad; Jawad Zahir; Shafiq Ur Rahman; Kaleem Ullah; Qazi Sami Ul Haq; Yasar Aziz; Jawad Khan; Sania

doi:10.70749/ijbr.v3i4.954

Authors

Waseem Sajjad Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad.
Fahim Ullah Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad.
Rizwan Ullah Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad.
Zia Ullah Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad.
Mudassir Ahmad Poultry Research Institute Rawalpindi (46000), Punjab, Pakistan.
Jawad Zahir Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad.
Shafiq Ur Rahman Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad.
Kaleem Ullah Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad.
Qazi Sami Ul Haq Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad.
Yasar Aziz Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad.
Jawad Khan Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad.
Sania Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad.

DOI:

https://doi.org/10.70749/ijbr.v3i4.954

Keywords:

Withania somnifera, Antibacterial Efficacy, Medicinal Properties, Bacterial Strains

Abstract

The overuse of chemically synthesized antibiotic drugs and the internal defense mechanism of microorganisms make the currently available antibiotics less efficient because the microbes have developed resistance against them... to handle such kind a solution, the necessity to find new antibacterial agents significantly increases the phytochemical substances present in nature have significantly less production cost and are environmentally friendly. The plant contains an inhibitory mechanism against many bacteria and can be the better alternative to chemically synthesized drugs because of their organic-based origin. The main aim of the current study was to analyze and assess the antimicrobial potential of the selected medicinal plants Withania somnifera against some pathogen bacteria such as coli Neisseria. The alcoholic extracts of the medicinal plants were prepared using a soxhlet apparatus. The antimicrobial potential of the extract was evaluated using the healthy diffusion method. As well as the disc diffusion method. The inhibition zones against every bacteria were recorded in diameter, taking millimeters as a measuring scale. Among the selected medicinal plant extracts, the ethanol and chloroform extract, Withania somnifera, showed the highest activity against all the tested pathogenic organisms. Among the pathogenic bacteria used in the study, salmonella appeared to be most susceptible to the Withania somnifera medicinal plant as they showed susceptibility against the selected plant extract. The result of this study was encouraging. Our result indicates that there is the possibility of using this plant extract in the treatment of bacterial infection. Clinical studies also need to check the potential toxic effects and effectiveness against the infection caused by these pathogenic bacteria.

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References

A catalogue of Nature-Based solutions for urban resilience. (2021). https://doi.org/10.1596/36507

Baimey, H. K., Hamamouch, N., & Kolombia, Y. A. (2020). Horticultural crops. BoD – Books on Demand.

Blows, W. T. (2016). The biological basis of mental health. Routledge.

Brightly, W. H., Hartley, S. E., Osborne, C. P., Simpson, K. J., & Strömberg, C. a. E. (2020). High silicon concentrations in grasses are linked to environmental conditions and not associated with C4 photosynthesis. Global Change Biology, 26(12), 7128–7143. https://doi.org/10.1111/gcb.15343

Dua, K., Mehta, M., De Jesus Andreoli Pinto, T., Pont, L. G., Williams, K. A., & Rathbone, M. (2021). Advanced drug delivery systems in the management of cancer. Elsevier. https://doi.org/10.1016/b978-0-323-85503-7.00029-8

Egbuna, C., & Hassan, S. (2021). Dietary phytochemicals: A Source of Novel Bioactive Compounds for the Treatment of Obesity, Cancer and Diabetes. Springer Nature.

Hamilton, A. J. (2018). Scale and the Incas. Princeton University Press.

Hanif, M. A., Nawaz, H., Khan, M. M., & Byrne, H. J. (2019). Medicinal plants of South Asia: Novel Sources for Drug Discovery. Elsevier.

Idžojtić, M. (2019). Dendrology: cones, flowers, fruits and seeds. Academic Press.

Khan, Z. H. (2017). Nanomaterials and their applications. Springer.

Kowalska, T., Sajewicz, M., & Sherma, J. (2015). Planar Chromatography - Mass Spectrometry. CRC Press.

Kumar, A., & Bagchi, D. (2021). Antioxidants and functional foods for neurodegenerative disorders: Uses in Prevention and Therapy. CRC Press.

Kumar, B., Bajpai, V., Gond, V., Tiwari, S., & Madhusudanan, K. P. (2022a). Phytochemistry of Withania somnifera.

Kumar, B., Bajpai, V., Gond, V., Tiwari, S., & Madhusudanan, K. P. (2022b). Phytochemistry of Withania somnifera.

Kumar, B., Bajpai, V., Gond, V., Tiwari, S., & Madhusudanan, K. P. (2022c). Phytochemistry of Withania somnifera.

Kumar, M., Muthusamy, A., Kumar, V., & Bhalla-Sarin, N. (2019). In vitro Plant Breeding towards Novel Agronomic Traits: Biotic and Abiotic Stress Tolerance. Springer Nature.

Macharia, M. J. (2023). Evaluation and determination of the phytotherapeutic properties of selected plants and their bioactive metabolites on targeted genes in colorectal cancer management. https://doi.org/10.15170/pte.2023.010

Nations, F. a. a. O. O. T. U. (2018). Crop ecology, cultivation and uses of cactus pear. Food & Agriculture Org.

Nations, F. a. a. O. O. T. U., Development, I. F. F. A., Programme, W. F., Organization, W. H., & Fund, U. N. C. (2020). The State of Food Security and Nutrition in the World 2020: Transforming food systems for affordable healthy diets. Food & Agriculture Org.

Nichols, G. (2005). Growing rare plants: A Practical Handbook on Propagating the Threatened Plants of Southern Africa.

Ozturk, M., & Hakeem, K. R. (2018). Plant and Human Health, Volume 1: Ethnobotany and Physiology. Springer.

Polumackanycz, M., Petropoulos, S. A., Śledziński, T., Goyke, E., Konopacka, A., Plenis, A., & Viapiana, A. (2023). Withania somnifera L.: Phenolic Compounds Composition and Biological Activity of Commercial Samples and Its Aqueous and Hydromethanolic Extracts. Antioxidants, 12(3), 550. https://doi.org/10.3390/antiox12030550

Rhoads, A. F., & Block, T. A. (2007). The Plants of Pennsylvania: An Illustrated Manual. University of Pennsylvania Press.

Roy, A. (2024). Harnessing medicinal plants in cancer prevention and treatment. IGI Global.

Sarris, J., & Wardle, J. (2019). Clinical naturopathy: An evidence-based guide to practice. Elsevier Health Sciences.

Swamy, M. K. (2020). Plant-derived bioactives: Production, Properties and Therapeutic Applications. Springer Nature.

Tripathi, K. (2018). Essentials of medical Pharmacology. Jaypee Brothers Medical Publishers.

Verma, D., Macwan, D., Patel, J. D., Parmar, S. R., & Patel, H. V. (2022). Herbs that heal: Role of traditional herbal remedies as an immunity booster and effective against the infectious and systemic diseases. Annals of Phytomedicine an International Journal, 11(2). https://doi.org/10.54085/ap.2022.11.2.2